Research Paper Volume 10, Issue 8 pp 2016—2036

Figure 4. Protective effect of lycopene on oxidative stress in aged ovarian tissues was similar to the effects of DMF. (A) Relative changes in the expression of Nrf2, pNrf2 and HO-1 after treatment with D-gal alone or combined with DMF or lycopene. (B) Changes in the morphology of ovarian tissues after treatment with D-gal alone or combined with DMF or lycopene, scale bar: 50 µm. (C and E) Changes in BrdU index in ovarian tissues after treatment with D-gal alone or combined with DMF or lycopene, scale bar: 20 µm. (D) Relative changes in the expression of proteins related to cell proliferation and cell apoptosis in ovarian tissues after treatment with D-gal alone or combined with DMF or lycopene. (E and G) Changes in TUNEL index in ovarian tissues after treatment with D-gal alone or combined with DMF or lycopene, scale bar: 20 µm. (F) Changes in ROS levels of the ovarian tissues after treatment with D-gal alone or combined with DMF or lycopene. Values are expressed as the means±s.e.. Different lowercase letters indicate significant differences (P < 0.05).