Research Paper Volume 10, Issue 3 pp 322—338

Figure 4B. High sensitivity to mild oxidative stresses exhibited by flies with EC-specific knockdown of DNA damage response (DDR)-related factors. EC-specific DDR-related factor knockdown flies evince a higher sensitivity to mild oxidative stress. Three-day-old flies carrying Myo^ts>GFP, Myo^ts>GFP+Mre11i, Myo^ts>GFP+Rad50i, Myo^ts>GFP+Nbs1i, Myo^ts>GFP+ATMi, Myo^ts>GFP+ATRi, Myo^ts>GFP+Chk1i, or Myo^ts>GFP+Chk2i genotypes were cultured for 6 h at 29 °C in normal media, and then were fed without (a-h, closed bars) or with 2 mM PQ (a’-h’, open bars) in 5% sucrose for 18 h at 29 °C. The number of PH3⁺ cells in their guts was imaged (a-h’) and counted (i). p-values were calculated using Student’s t-test. *p < 0.05.