Research Paper Volume 10, Issue 3 pp 322—338

Figure 1B. EC-specific knockdown of DDR cause EC cell death. EC-specific knockdown of Mre11, Rad50, Nbs1, ATM, ATR, Chk1, or Chk2 induce Cleaved caspase-3 in EC. Flies carrying Myo^ts>GFP (a-a’), Myo^ts>GFP+Mre11i (b-b’), Myo^ts>GFP+Rad50i (c-c’), Myo^ts>GFP+Nbs1i (d-d’), Myo^ts>GFP+ATMi (e-e’), Myo^ts>GFP+ATRi (f-f’), Myo^ts>GFP+Chk1i (g-g’), or Myo^ts>GFP+Chk2i (h-h’) genotypes were cultured at 29 °C for 4 days. a-h panels depict the merged images and a’-h’ panels denote the gray scale versions for the images corresponding to Cleaved caspase-3 signals. Original magnification is 400×. (i) A graph showing the ratio of Cleaved caspase-3⁺ cells in Myo-GFP⁺ cells. The data (mean ± SE) from 5 guts, respectively. ***p < 0.0001. The guts of flies were dissected and labeled with anti-GFP (green) and anti-Cleaved caspase-3 (red) antibodies and DAPI (blue).