Research Paper Volume 10, Issue 3 pp 322—338

Figure 1A. EC-specific knockdown of DDR cause EC cell death. Effect of knockdown of EC-specific DDR-related factors on γH2AvD signals after irradiation. γH2AvD signals after 5 Gy irradiation in the EC-specific Mre11, Rad50, Nbs1, ATM, ATR, Chk1, or Chk2 knockdown in the midgut. Flies carrying Myo^ts>GFP (a-a’ and i-I’), Myo^ts>GFP+Mre11i (b-b’ and j-j’), Myo^ts>GFP+Rad50i (c-c’ and k-k’), Myo^ts>GFP+Nbs1i (d-d’ and l-l’), Myo^ts>GFP+ATMi (e-e’ and m-m’), Myo^ts>GFP+ATRi (f-f’ and n-n’), Myo^ts>GFP+Chk1i (g-g’ and o-o’), or Myo^ts>GFP+Chk2i (h-h’ and p-p’) were cultured at 29 °C for 2 days and exposed to γ-radiation. a-h’, non-irradiation. i-p’, 5 Gy irradiation. a-p panels depict the merged images and a’-p’ panels denote the gray scale versions for the images corresponding to γH2AvD signals. One hour after irradiation, the guts of the irradiated flies were dissected and labeled with anti-GFP (green) and anti-γH2AvD (red) antibodies and 4′,6-diamidino-2-phenylindole (DAPI, blue). Yellow arrows indicate EC. Original magnification is 400×.