Research Paper Volume 9, Issue 12 pp 2471—2479
MicroRNA-939 inhibits cell proliferation via targeting LRSAM1 in Hirschsprung’s disease
Figure 3. Mir-939 directly regulates LRSAM1. (A) Sequence alignment of human miR-939 with 3’UTR of LRSAM1. Bottom: mutations in the 3’-UTR of LRSAM1. (B) All cells were transfected with miR-939 and negative control, renilla luciferase vector pRL-SV40 and LRSAM13’UTR luciferase reporters. Both firefly and Renilla luciferase activities are measured in the same sample. Firefly luciferase signals were normalized with Renilla luciferase signals. *indicates significant difference compared with control group, P<0.05.