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Research Paper Volume 9, Issue 7 pp 1825—1843

Aging and calorie restriction regulate the expression of miR-125a-5p and its target genes Stat3, Casp2 and Stard13

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Figure 5. mIR125a-5p targets 3’UTR of Stard13. (A) luciferase reporter construct containing 228 nucleotide sequence from Stard13 3’ UTR with either WT or mutant (MUT) miR125a-5p target sites. (B) CHO-K1 cells were cotransfected with the constructs presented in figure 5(A) and with mmu-miR 125a-5p mimic or Negative control (NC) as indicated.