Research Paper Volume 9, Issue 5 pp 1359—1374

Figure 5. Neutrophils are required for inhibiting LPC proliferation in old mice. Old mice with CDE diet were i.p. injected with 500 μg of 2A3 (n/a) or 1A8 (anti-mLy6G) mAb every other day to deplete neutrophils. (A) Representative FACS scatterplots of liver-infiltrating neutrophils (CD11b⁺Gr-1^high) in liver tissues from O-CDE mice treated with 2A3 or 1A8 mAb. Percentages of liver-infiltrating neutrophils were quantified. Results are mean ± SEM from three independent experiments (n = 3 mice per group). (B) Cumulative survival rates of mice were analyzed. n > 8 mice per group. (C) Representative FACS scatterplots and summarized percentages of EpCAM⁺CD45^- cells in liver tissues from O-CDE mice treated with 2A3 or 1A8 mAb. Results are mean ± SEM from three independent experiments (n > 5 mice per group). *P < 0.05, **P < 0.01.