Research Paper Volume 9, Issue 4 pp 1153—1185

Figure 12. Hydrogen peroxide stress resistance improves with continual knockdown of Keap1 in aged (60 days) flies. In panels A and B, male and female progeny of the Actin-GS-255B strain crossed to Keap1 RNAi strain were collected and aged to 60 days. (A) Females raised in the absence of RU486 were either pretreated with 10µM H₂O₂ (gray line) “10µM H₂O₂” or not pre-treated with H₂O₂ (black line) “No Additions”. Females raised in the presence of RU486 were either pretreated with 10µM H₂O₂ (red line) “10µM H₂O₂ (+RU486)” or not pretreated with H₂O₂ (pink line) “+RU486”. (B) Males raised in the absence of RU486 were either pretreated with 10µM H₂O₂ (gray line) “10µM H₂O₂” or not pre-treated with H₂O₂ (black line) “No Additions”. Males raised in the presence of RU486 were either pretreated with 10µM H₂O₂ (blue line) “10µM H₂O₂ (+RU486)” or not pretreated with H₂O₂ (green line) “+RU486”. Statistical difference in survival (p < 0.05) was calculated using the Log-Rank test. Statistical summary is located in Supplementary Table S4.