Research Paper Volume 9, Issue 4 pp 1153—1185

Figure 11. Loss of proteasomal subunits or regulators impacts lifespan. (A,B) To control for the effect of RU486 on males and females, lifespan of progeny from the Actin-GS-255B strain crossed to w[1118] strain raised in the absence/control (black line) or presence of RU486, pink line for females and blue line for males. (A) Females. (B) Males. (C-F) Effect of removal of proteasome subunits on life span. The Actin-GS-255B strain was crossed to the β₁ RNAi or β₂ RNAi strains and the progeny were assayed for life span in the absence/control (black line) or presence (pink line in females and blue line in males) of RU486, as indicated. (C) β₁ RNAi females. (D) β₁ RNAi males. (E) β₂ RNAi females. (F) β₂ RNAi males. (G,H) Effect of removal of the Cap-n-collar (CncC)/Nrf2 orthologue upon lifespan. The Actin-GS-255B strain was crossed to the CncC RNAi strain. Male and female lifespan was measured in the absence/control (black line) or presence (pink line for females and blue line for males) of RU486. (G) CncC RNAi female. (H) CncC RNAi male. (I,J) Effect of removal of Keap1 upon lifespan. The Actin-GS-255B strain was crossed to the Keap1 RNAi strain. Males and female lifespan was assessed in the absence/control (black line) and presence (pink line for females and blue line for males) of RU486. (I) Keap1 RNAi female. (J) Keap1 RNAi male. Statistical difference in survival (p < 0.05) was calculated using the Log-Rank test. Statistical summary is located in Supplementary Table S3