Figure 8.(A) Western blot showing that BMP2, annexin A6 and annexin A2 carriage to be higher in MVs from senescent than from young HUVEC. Equal protein loading was confirmed using a HSP90 antibody. Total protein expression was obtained via densitometric analysis and expressed as the ratio protein/HSP90 in arbitrary units. n=3 pools; *p<0.05; **p<0.01. (B) Representative BMP2, annexin A6 and annexin A2 western blot of young and senescent HUVEC pools. Equal protein loading was confirmed probing with GAPDH or β-Actin. The graphs present densitometric band analysis normalized to GAPDH or β-Actin in arbitrary units. n=3 pools.
