Research Paper Volume 8, Issue 12 pp 3486—3497
Proteins that accumulate with age in human skeletal-muscle aggregates contribute to declines in muscle mass and function in Caenorhabditis elegans
Figure 1. The protein content of muscle aggregates from older subjects was higher than that from young adults. Sarcosyl-insoluble aggregates were isolated from muscle of old (A) and young (B) donors (pooled, N=2 per group), dissolved in ampholyte buffer, resolved on 2D gels, and stained for total protein. Aggregates from 5 individuals per group were resuspended and heated to 95oC in Laemmli buffer (containing 2% SDS, w/v, and 0.5% ß-mercaptoethanol, v/v), and resolved on 1D gels to quantify total aggregate protein (C). Mean ±SD is shown after normalization to mean total aggregate protein for the young group.