Research Paper Volume 8, Issue 11 pp 2988—3008

Figure 4. Transgenerational alleviation of the LP-induced longevity decrease after post-eclosion inhibition of the E(z) enzymatic function in F0 parents with EPZ-6438. (A−B) Survival curves for virgin males, and (C−D) H3K27me3 level in the F0 parents (A and C) and their F2 offspring (B and D). The F0 parents were subjected to the 7-day PDM with the LP diet (in red) along or LP diet containing EPZ-6438 at the concentration of 150 μM (LP+150μM; in green) and with CD as the control (in black). N=96−99 for longevity analyses, and N=4 for western analyses of H3K27me3. The asterisk (*) indicates a significant difference from control (see Table S3 for detailed analyses and specific P values).