Figure 3. Transgenerational longevity extension and H3K27me3 downregulation after post-eclosion inhibition of the E(z) enzymatic function in the F0 parents. (A−B) Survival curves for virgin males, (C−D) E(z) protein level, and (E−F) H3K27me3 level in the F0 parents (A, C and E) and their F2 offspring (B, D and F). All the flies were raised on CD at all times after EPZ-6438 feeding dissolved in 5% sucrose as vehicle. Inhibition of the E(z) methyltransferase activity was induced for 7 days following eclosion in a dose-dependent manner. The doses of 100, 200 and 400 μM were first used to determine an effective dose at 100 μM through western analyses of H3K27me3 (see