Research Paper Volume 8, Issue 11 pp 2988—3008

Figure 2. Transgenerational longevity extension and H3K27me3 downregulation after specific post-eclosion RNAi-mediated KD of the E(z) gene in the F0 parents. (A−B) Survival curves for virgin males, (C−D) E(z) protein level, and (E−F) H3K27me3 level in the F0 parents (A, C and E) and their F2 offspring (B, D and F). All the flies were raised on CD at all times. Specific RNAi-mediated KD of E(z) was induced twice per day via heat shock (25 → 37°C for 1 hour) for 7 days immediately after eclosion. Two independent lines were used (33659, in green; or 27993 in purple), with their parental line as the control (Attp2, in black). Genotypes: Attp2: F0 — HS-Gal4; Attp2 / +; +, 33659: F0 — HS-Gal4; 33659 RNAi / +; +, 27793: F0 — HS-Gal4; 27793 RNAi / +; +, Attp2: F2 (+/+) — +; + / +; +, 33659: F2 (+/+) — +; + / +; +, 27793: F2 (+/+) — +; + / +; +. N=145−149 for longevity analyses, and N=4 for western analyses of E(z) and H3K27me3. The asterisk (*) indicates a significant difference from control (see Table S1 for detailed analyses and specific P values).