Research Paper Volume 8, Issue 11 pp 2927—2935

Figure 1. Determination of expression level and localization of MDH1 in brain tissue of CJD patients. (A) MDH1 was detected by western blot analysis in the frontal cortex region 8 of sCJD patients (n=8) in comparison to controls (n=8). Densitometric analysis of band intensities revealed a lower of MDH1 level in sCJD patients (MM1 and VV2). (B) Immunohistochemical staining using anti-MDH1 confirmed the decreased expression of MDH1 in sCJD (MM1) patients (n=5) compared to age-matched controls (n=4). For comparison between two groups we used the Wilcoxon Mann Whitney test. A p-value < 0.001 is considered as extremely significant (***), < 0.01 as very significant (**), < 0.05 as significant (*) and ≥ 0.05 as not significant (ns).