Research Paper Volume 8, Issue 11 pp 2915—2926
Discovery of piperlongumine as a potential novel lead for the development of senolytic agents
Figure 1. Senolytic activity of piperlongumine (PL). (A) Structures of PL, 2,3-dihydro-PL, and 7,8-dihydro-PL. (B) Quantification of viable WI-38 non-senescent cells (NC), IR-induced senescent cells (IR-SC), replication-exhausted senescent cells (Rep-SC), or Ras-induced senescent cells (Ras-SC) 72 h after treatment with increasing concentrations of PL (n = 3). (C) Quantification of viable IR-SCs over time after treatment with 10 µM PL (left) or after incubation with 10 µM PL, removal of the drug, and further culture for 72 h (right) (n = 3). (D) Quantification of viable WI-38 NCs and IR-SCs 72 h after treatment with increasing concentrations of 2,3-dihydro-PL or 7,8-dihydro-PL (n = 3). Data are represented as the mean ± SEM.