Research Paper Volume 8, Issue 11 pp 2790—2798

Expression of Rab1A is upregulated in human lung cancer and associated with tumor size and T stage

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Figure 3. Rab1A does not regulate mTOR signaling or cell growth in lung cancer. (A) The effects of Rab1A knockdown was examined in H838, H1975, H650 and A549 cells by immunoblot. Levels of P-S6K1 (T389), S6K, P-AKT (S473) and AKT are shown. β-tubulin was used as a loading control. (B) Rab1A was knocked down in H838, H1975, H650 and A549 cells. The relative growth of these cells was analyzed using an MTT assay. Data represent means ± SD of three independent triplicate experiments. si-NC, negative control siRNA; si-Rab1A, Rab1A-specific siRNA.