Research Paper Volume 8, Issue 11 pp 2754—2776
Aging is associated with an expansion of CD49fhi mammary stem cells that show a decline in function and increased transformation potential
Figure 2. Stem cell function and transformation potential in young and old mammary glands. (a) Immunohistochemistry staining of β-casein showing more aveoli with light staining of β-casein in regenerated outgrowths derived from old MaSCs (26-29 mon.) than in those derived from young MaSCs (7-9 mon.) isolated from DsRed-C57BL6/J donor mice (n = 3 paired regenerated outgrowths from young and old MaSCs injected in the right or left side of the same recipient mice; paired t-test). (b) Frequency of preneoplastic lesions (expressed as % hyperplasia) in primary and MaSC-regenerated glands from young (7-9 mon.) and old (26-29 mon.) DsRed-C57BL6/J mice. (c) Frequency of preneoplastic lesions (expressed as % hyperplasia) and neoplastic lesions (% atypical ductal hyperplasia/ductal carcinoma in situ [ADH/DCIS]) in primary and MaSC-regenerated glands from young (2-4 mon.) and old (17-27 mon.) BALB/c mice.