Research Paper Volume 8, Issue 9 pp 2006—2022

Figure 4. SATB2-modified BMSCs prevent alveolar bone loss of aging rats. (A) The region of molar furcation in rats mandible demonstrated a significantly reduced bone mass with aging. (B) Bone histomorphometric parameters revealed a decrease in BV/TV, (C) Tb.N., (D) Tb.Th. and (E) an increase in Tb.Sp. (F) BMSCs from rats mandible showed a declined colony-forming units with aging. (G) Western blots showed the decrease of SATB2, Nanog, SOX2 and OCT4 expression with aging. (H) SA-β-Gal positive cells and senescence-related factors P16, P21 and P53 (I) increased with aging. (J) HE staining demonstrated abundant trabecular bone in Lev-satb2 group compared with Lev-GFP group (alveolar bone showed in yellow line). (K-O) Histomorphometric analysis of alveolar trabecular bone after treatment with BMSCs, representative micro-CT images (K), BV/TV (L), Tb.N. (M), Tb.Sp (O), and Tb.Th(N) between Lev-GFP group and Lev-satb2 group. #p> 0.05, *p< 0.05, **p< 0.01, ***p< 0.001. Scale bar = 100 μm.