Research Paper Volume 8, Issue 9 pp 2153—2181
Age-related changes in cerebellar and hypothalamic function accompany non-microglial immune gene expression, altered synapse organization, and excitatory amino acid neurotransmission deficits
Figure 7. Vglut1 and C3 show increased expression in the cerebellar internal granule cell layer of the aged C57BL/6 mouse and in the hypothalamic arcuate nucleus of the aged BALB mouse. (A) C57BL/6 cerebellar internal granule cell layer. a DAPI stain, young. b Vglut1 immunoreactivity, young. c. C3 immunoreactivity, young. d Merge, young. Note significant colocalization of Vglut1 and C3 staining, particularly for more intense puncta. e DAPI, aged. f Vglut1, aged. g C3, aged. h Merge, aged. Bottom: Quantification of Vglut1, C3, and DAPI. (B) BALB hypothalamic arcuate nucleus. Panels a-h as above. Again, note significant colocalization of Vglut1 and C3 staining, particularly for more intense puncta. Asterisk denotes p<0.01. Scale bar 4 μm.