Research Paper Volume 8, Issue 11 pp 2667—2688

Figure 7. BubR1 insufficiency causes reduction in dendrite spine density of motor neurons and cerebellar purkinje cells. (A) Motor neuron density in lumbar spinal cord is not significantly different between WT and BubR1^H/H mice. Left: Representative image of spinal cord with Nissl staining. Right: Quantification of lumbar motor neuron number. Scale bar: 500 µm. (B) Neuron density in primary motor cortex is not significantly different between WT and BubR1^H/H mice. Left: Representative image of NeuN (a neuronal marker) and DAPI staining of motor cortex neurons. Right: Quantification of NeuN⁺ cell density. Scale bar: 50 µm. (C) BubR1^H/H mouse motor cortex neurons exhibit a reduced dendritic spine density. Left: Representative image of golgi-stained motor cortex neuron dendritic spines. Right: Quantification of dendritic spine density. Scale bar; 1 µm. (D) BubR1^H/H mouse cerebellar Purkinje neuron density is not significantly different from WT mice. Left: Representative image of Calbindin (marker for Purkinje cells) and DAPI staining of Purkinje neurons. Scale bar: 20 µm. Right: Quantification of Calbindin⁺ cell density. (E) BubR1^H/H mouse cerebellar Purkinje neurons exhibit a reduced dendritic spine density. Left: Representative image of golgi-stained Purkinje neuron dendritic spines. Right: Quantification of dendritic spine density. Scale bar; 1 µm. All values represent mean ± SEM (ns: non-significant, *P < 0.05, **P < 0.01, two-tailed student’s t-test). Number associated with bar graphs indicates number of animals examined.