Research Paper Volume 8, Issue 11 pp 2667—2688

Figure 2. BubR1 insufficiency impairs OPCs proliferation during oligodendrocyte development in the CNS. (A-B) BubR1 expression in isolated primary oligodendrocyte progenitor cells. Representative image of NG2⁺ cell (a marker for oligodendrocyte progenitor cells) (A). Representative image of Olig2⁺ cell (a marker for oligodendrocyte lineage cells) (B). Scale bar: 25 µm for (A) and (B). (C-D) The number of proliferating oligodendrocyte lineage cells in the corpus callosum. (C) Representative images of MCM2⁺Olig2⁺ cells within the corpus callosum at 1, 2, 4, and 8 weeks old. (D) Quantification of MCM2⁺Olig2⁺ cell number in the corpus callosum. Scale bar: 50 µm. (E,F) The number of proliferating oligodendrocyte lineage cells in the white matter of spinal cord. (E) Representative images of MCM2⁺Olig2⁺ cells in the white matter of spinal cord at 1, 2, 4, and 8 weeks old. Scale bar: 50 µm. (F) Quantification of proliferating OPCs (Olig2⁺MCM2⁺) in the white matter of spinal cord. All values represent mean ± SEM (ns: non-significant, *P < 0.05, **P < 0.01, student’s t-test). Number associated with bar graphs indicates number of animals examined.