Research Paper Volume 8, Issue 11 pp 2667—2688

Figure 1. Morphological characterization of BubR1 insufficient mice. (A-C) Reduced size of BubR1^H/H mouse brain. (A) Representative photographs of WT and BubR1^H/H mice at postnatal day 7 (P7) and quantification of brain weight. Scale bar: 0.5 cm. (B) Representative photographs of WT and BubR1^H/H mice at postnatal day 56 (P56; 8-week-old). Scale bar: 0.5 cm. BubR1^H/H exhibit a significantly reduced brain size including rostrocaudal (R-C) length, and width (L-R) (B) and weight (C). (D-F) Deficits in corpus callosum formation in BubR1 insufficient mice. (D) Representative images of quantification of cortex and corpus callosum area relative to total section area using cresyl violet stained sections. The cortex is highlighted in dark blue, the corpus callosum is highlighted in yellow. Measurements of the corpus callosum, and cortex area. Arre of the corpus callosum in BubR1 insufficient mouse is significantly different from WT mice (E), while cortical area is not (F). All values represent mean ± SEM (ns = non-significant, **P < 0.01, ***P < 0.001, student’s t-test). Number associated with bar graphs indicates number of animals examined.