Research Paper Volume 8, Issue 9 pp 1979—2005

Figure 3. mir-60 mutants exhibit features that are commonly observed in long-lived mutants. (A) The number of progeny derived from wild-type and mir-60 mutant parents was examined. Error bars represent SE calculated from the results of 8 individual parents in each strain. P-values were calculated by unpaired t-test: ***p<0.001, and ‘ns’ means not significant with p=0.1 or higher. The experiments were repeated 4 times, including 25 parents in total for each strain, and essentially the same results were obtained (data not shown). (B) Body size was examined using microscopic images of approximately 50 individuals for each strain/day and shown as each dot. P-values were calculated by unpaired t-test: ***p<0.001. (C) The box plot represents the distribution of age pigments. a.u. denotes arbitrary unit. Approximately 25 individual animals were examined at each time point. Signal intensities were normalized by the body size in individual animals. Unpaired t-test was used to calculate p-values (compared to wild-type control at each day examined): ***p<0.001. In this assay, animals were treated with PQ 5 mM during adulthood to enhance the effect of the mir-60 loss on lifespan. Representative images having average pigment intensities are shown in (D) for Day 0 and Day 4 animals.