Research Paper Volume 8, Issue 9 pp 1979—2005

Figure 1. C. elegans miR-60 shows a specific spatio-temporal expression pattern. (A) Fluorescent signals from miR-60::GFP, which are exclusively localized in the intestine, are shown. Approximately 25 individuals of (B) mir-60::gfp;glp-4 and (C) mir-243::gfp;glp-4 strains each were used for measuring GFP intensity at each time point examined, and the measurement for each individual is represented by a dot. Both strains were cultured at a restrictive temperature 23.5 °C to induce germline deficiency. a.u. denotes arbitrary unit. Representative images of the mir-60::gfp;glp-4 strain, which are close to the average signal intensity, are shown in (A). (D) A bar graph represents the relative expression of mature miR-60 (miR-60-3p strand) during normal aging in temperature-sensitive sterile mutants spe-9(hc88) cultured at 23.5 °C (see Supplemental File for spe-9 mutants). Error bars represent the range in the results of 2 biological replicates, in which the total RNA was purified from 2 independent experimental trials.