Research Paper Volume 8, Issue 2 pp 402—417

Figure 4. Sir2 mRNA levels increase with D. pulicaria age. Total RNA was harvested from groups of 15 D. pulicaria at each age. Real-time PCR was performed using a BioRad CFX 96 using Sir2-specific primers and GAPDH primers as a normalization control. Data was analyzed using the 2^−ΔΔCt method and are represented as relative Sir2 mRNA levels (green bars). Error bars represent standard deviations. The ages are as indicated, wk: weeks. To analyze statistical significance, an ANOVA was performed (F: 145.95, df: 2,6, p:1.38×10⁻⁷) followed by a post hoc Tukey test. The post hoc analysis revealed that all three means were statistically different from one another and for all: * (statistical significance between 1 wk and 4 wk), ** (statistical significance between 1 wk and 8 wk), and *** (statistical significance between 4 wk and 8 wk) and indicate p<0.05.