Research Paper Volume 8, Issue 2 pp 366—381
Permanent farnesylation of lamin A mutants linked to progeria impairs its phosphorylation at serine 22 during interphase
Figure 2. Intranuclear progerin foci contain serine 22 phosphorylated progerin. (A) Immunoblots for phosphorylated lamin A at serines 22 and 392 in U-2 OS cells expressing progerin (Prog) or progerin CS (ProgCS). (B) Immunofluorescence for phosphoserine 22 lamin A (p-S22Lam) in U-2 OS cells expressing progerin or progerin CS. Magnification = 10 μm. (C) Immunoblot for phosphoserine 22 lamin A in U-2 OS cells expressing progerin and treated with 3 μM FTI-277 or vehicle. (D) Immunofluorescence for phosphoserine 22 lamin A in cells expressing progerin and treated with 3 μM FTI-277 or vehicle. (E) Colony assays of U2-OS cells expressing the indicated vectors and treated with 3 μM FTI-277 or vehicle. (F) Quantification of the colony assay in (E).