Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes

María Eugenia Matzkin; Johanna Gabriela Miquet; Yimin Fang; Cristal Monique Hill; Daniel Turyn; Ricardo Saúl Calandra; Andrzej Bartke; Mónica Beatriz Frungieri

doi:doi:10.18632/aging.100875

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Research Paper Volume 8, Issue 1 pp 95—110

Alterations in oxidative, inflammatory and apoptotic events in short-lived and long-lived mice testes

Figure 6. Longevity is inversely associated with testicular apoptosis. Testicular expression of pro-caspase 3 (32 kDa) and cleaved caspase 3 (15 kDa) was measured by immunoblotting in testicular homogenates of short-lived (GH-Tg) and long-lived (GHRH-KO and Ames dwarf) mice and their normal littermates. Bar plot graphs represent the mean ± S.E.M. and depict the quantification by densitometry of the bands. Results were normalized to actin (42 kDa) and expressed as fold change relative to the control (normal littermates), which was assigned a value of 1. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test.