Research Paper Volume 8, Issue 1 pp 95—110

Figure 1. Longevity is inversely associated with testicular COX2 expression and PGD2 production. Panel (A) COX2 (72 kDa) expression was evaluated by immunoblotting in testicular homogenates of short-lived (GH-Tg) and long-lived (GHRH-KO and Ames dwarf) mice. Bar plot graphs represent the mean ± S.E.M. and depict the quantification by densitometry of the bands. Results were normalized to actin (42 kDa) and expressed as fold change relative to the control (normal littermates), which was assigned a value of 1. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test. Panel (B) PGD2 testicular levels were determined by immunoassay in testicular homogenates from short-lived (GH-Tg) and long-lived (GHRH-KO and Ames dwarf) mice. Bar plot graphs represent the mean + SEM; n = 5-7. * p < 0.05, t-Student test.