Figure 3. Early or late glucose enrichment has opposite effects on lifespan. (a) GE reduced lifespan of N2 animals at 4% concentration compared to untreated animals (P<0.0001 compared to control). (b) Larval exposure to glucose extends lifespan of N2 animals at concentrations of 2% or 4% compared to untreated animals (P<0.0001 compared to controls). (c) Adult exposure to glucose reduces lifespan of wild-type animals (P<0.0001 for all conditions compared to control). (d-e) GE failed to extend lifespan in atfs-1(et15) and atfs-1(gk3094) animals when treatment ceased at the L4 larval stage. (f) GE failed to extends lifespan in N2 animals when treated with 10 mM N-acetyl cysteine.