Research Paper Volume 6, Issue 12 pp 1049—1063

Figure 6. Progerin expression significantly alters membrane stiffness when measured by atomic force microscopy. (A,D) Progerin expression significantly increases membrane stiffness in both cytoplasmic (A) and nuclear (D) regions when compared to control cell lines, while stiffness in cytoplasmic and nuclear regions are not significantly different between control cell lines. (B, E) FTI treatment significantly decreases membrane stiffness in cytoplasmic (B) and nuclear (E) regions in progerin-MIAMI cells when compared to untreated progerin-MIAMI cells, while stiffness in cytoplasmic and nuclear regions are not significantly different before and after FTI treatment in control cell lines. (C, F) FTI treatment significantly decreases membrane stiffness in cytoplasmic (C) and nuclear (F) regions in progerin-MIAMI cells to levels that are significantly less than treated control cell lines, while stiffness in cytoplasmic and nuclear regions are not significantly different between control cell lines after FTI treatment. Values are mean ± standard deviation (n≥3). *p<0.05, **p<0.01, calculated by Student's t-test.