Research Paper Volume 6, Issue 12 pp 1094—1108

Aged iPSCs display an uncommon mitochondrial appearance and fail to undergo in vitro neurogenesis

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Figure 2. Analysis of neuronal differentiation and cell death in y- and a-iPSCs. Immunofluorescence analysis of y-iPSCs (A) and a-iPSCs (B) during neuronal differentiation stained with the neuronal marker β–III TUBULIN with nuclei counterstained with Hoechst. On the right of each photographs, the TUNEL assay corresponding to the same experiment is reported. The green cells represent TUNEL positive cells. Scale bar: 30 μm.