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Research Paper Volume 6, Issue 9 pp 771—786

Age- and glycemia-related miR-126-3p levels in plasma and endothelial cells

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Figure 6. Relative miR-126-3p expression and SPRED1-protein levels in intermediate age HUVECs undergoing senescence in normoglyacemic and hyperglycemic conditions. Intracellular miR-126-3p and miR-126-3p recovered from medium in normoglycemic (NG) (a) and hyperglycemic (HG) cultures (b). SPRED1-protein levels in normoglycemic (NG) and hyperglycemic (HG) cultures (c). Hyperglycemic medium contained 25 mM glucose. Mannitol was used as an osmotic control in the normoglycemic cultures. MiR-126-3p is expressed as 2ΔΔΔCt normalized with RNU44. * GLM, p<0.05.