Research Paper Volume 6, Issue 9 pp 742—754
Rapamycin-induced metabolic defects are reversible in both lean and obese mice
Figure 4. (A) Representative blots of p-S6, total S6, pAkt (Ser473) and total Akt in skeletal muscle and adipose of mice fed low fat or high fat diets with or without encapsulated rapamycin (eRAPA). (B) Quantification of relative phosphorylation of S6 or Akt (Ser473) of blots in A. Bars represent average (± SEM) values for n=4 mice treated with (solid) or without (open) eRAPA. Asterisks indicate significant difference between control and eRAPA group. (C) Representative blot of immunoprecipitation of mTOR from skeletal muscle protein homogenates and probed for the indicated proteins.