Research Paper Volume 6, Issue 1 pp 70—81
Serines 440 and 467 in the Werner syndrome protein are phosphorylated by DNA-PK and affects its dynamics in response to DNA double strand breaks
Figure 1. Mapping DNA-PK phosphorylation sites in WRN. (A) Schematic representation of His- or GST-tagged WRN fragments used in in vitro phosphorylation assay. (B and C) In vitro phosphorylation assay. Purified His- or GST-tagged WRN fragments were incubated with purified DNA-PKcs, Ku 70/86, and activated DNA in the presence of [γ-32P]ATP. Amido black staining is shown (B). The phosphorylation was visualized (C). Asterisk indicates the GST (500-946) fragment. Note that GST (239-499) migrated slower because of many acidic amino acids.