Research Paper Volume 5, Issue 11 pp 835—849
SIRT4 regulates ATP homeostasis and mediates a retrograde signaling via AMPK
Figure 5. Sirt4 deficiency initiates a homeostatic feedback loop via ANT2/AMPK. (A) Western blots for p-AMPK, AMPK, Sirt4-FLAG and actin in control and Sirt4OE HEK293T cells. (B) Western blots for p-AMPK, AMPK and actin in control and Sirt4KD HEK293T cells. (C) Western blots for p-AMPK, AMPK, Sirt4 and tubulin in liver lysates from wild-type and Sirt4KO mice under fed conditions. (D) Western blots for p-AMPK, AMPK and actin in control and Sirt4OE HEK293T cells in the presence or absence of ANT2 (+/− ANT2-siRNA), as indicated. (E) Western blots for p-ACC, ACC, Sirt4 and actin in liver lysates from wild-type and Sirt4KO mice under fed conditions. (F) Western blots for PGC1α and actin in control and Sirt4OE or Sirt4KD HEK293T cells, as indicated. (G) Western blots for PGC1α and actin in liver lysates from wild-type and Sirt4KO mice under fed conditions.