Research Paper Volume 5, Issue 9 pp 643—652

Figure 5. Increased proliferation and myeloid differentiation of WT progenitor cells. Lineage⁻ hematopoietic progenitor cells were isolated from 12 months old WT and CD137L^−/− mice, labeled with CFSE, and cocultured with activated WT CD4⁺ T cells at a ratio of 1:2 for 6 days. Cells were then harvested and stained for CD4, CD11b, Ly6G and Ly6C. (A) Gating strategy of identifying CD4⁺ T cells and CFSE-labeled progenitor cells. (B) Percentages and (C) absolute numbers of CD11b⁺, Ly6G⁺ and Ly6C⁺ cells in the CFSE⁺ population. Quadruplicates were performed for each treatment group. Data are representative of two independent experiments. *p<0.05.