Research Paper Volume 5, Issue 2 pp 130—141

Figure 1. Examples of novel circulating miRNAs discovered with mouse serum small RNA sequencing. (A) A novel miRNA located in a conserved genomic region and predicted to map to an intron of the Gnb2 gene as annotated in the RefSeq Genes Track. Shown are screenshots from the UCSC genome browser, displaying the Illumina sequencing reads (red), and the novel precursor miRNA (blue) predicted by miRDeep2 with a provisional id chr5_12913 (see Table S1). (B) A novel miRNA with a provisional id chr4_8812 (see Table S1) predicted to map to a genomic region with no known annotated features. UCSC genome browser Ensembl and RefSeq Genes tracks are shown, with no RNAs annotated in the genomic region of this novel miRNA. A miRBase v.19 custom track was uploaded to the UCSC genome browser to show absence of known miRNAs in the genomic regions of the predicted novel miRNAs. The “stacks” of sequence reads identify the mature miRNA. The coverage depth (number of reads, y-axis) shows fewer reads mapping to the star region of the miRNA precursor. The mammalian conservation track is at the bottom (green).