Research Paper Volume 4, Issue 5 pp 368—374
Integrin α5β1 simultaneously controls EGFR-dependent proliferation and Akt-dependent pro-survival signaling in epidermoid carcinoma cells
Figure 1. Efficient shRNA-mediated inhibition of a5 in A431 cells. Cells were transduced with lentivirus, containing #49 or #53 PLKO.1 plasmid vectors with a5 shRNAs, or empty (control) vector as described in “Materials and methods”. (A) Western blot analysis of cell lysate proteins. The blots were probed with 1:1000 dilution of polyclonal α5 antibody and 1:500 dilution of β-actin mAb, stained using ECL system and scanned. (B and C) Flow cytometry of the cells tranduced with the empty (control) PLKO.1 vector and stained with FITC-labeled anti-mouse IgG (B) or with anti-α5β1 mAb and FITC-labeled anti-mouse IgG (C). (D and E) flow cytometry of the cells tranduced with the α5 shRNA PLKO.1 vectors #53 and #49, respectively, and stained as in C.