Figure 9. Tyrosine nitration of GAPDH in plantaris/FHL lysate from old mice. (A) Tyrosine nitration was assessed by Western blot of one dimensional gel electrophoresis. (B) Densitometric analysis shows nitration was significantly increased for GAPDH at 1, 3, and 5 days of reperfusion. C = contralateral control Is = I/R; Group I = 1 day reperfusion; Group II = 3 days reperfusion; Group III = 5 days reperfusion, and Group IV = 7 days reperfusion. * = p < 0.05.