Figure 5. Age-dependent changes in ratio of acetylated to total p53 in blood and brain samples of C57/B6 mice. Western blot analysis of acetylated p53 proportion in age groups from 2 days to 25 months; Panels A, B, and C show acetylated P53 proportion normalized with Ponceau S in plasma (Panel A), and with β-actin in PBMCs and brain samples, along with histograms presenting the average densitometric values. Panel D presents a composite graph of the percentage of acetylated P53 expression in the total p53 protein pool in plasma, PBMC and brain samples. (*p < 0.01, **p < 0.0001; all histograms represent Mean ± SD; n = 3; three different biological samples from each age group)