Research Paper Volume 3, Issue 10 pp 985—1002

Figure 3. Expression of miRNA-34a and SIRT1 in brain tissue sections from middle and old age C57/B6 mice. In situ hybridization detected miR-34a expression using LNA probes. Panels (A) and (B) show expression of miR-34a in cortex and hippocampus of 10 and 31 month old mice. Scrambled LNA probes were used as control for this experiment (Image not shown); (C) graphical presentation of mean intensity values of miR-34a expression in cortex and hippocampus of 10 and 31 month old mice, by immunostaining assay to detect SIRT1 expression. Panels (D-E) and (F-G) show expression of SIRT1 in cortex and hippocampus of 10 and 31 month old mice, by DAPI staining to detect nucleus and section integrity (Image not shown); (H) graphical presentation of mean intensity values of SIRT1 expression in cortex and hippocampus of 10 and 31 month old mice. (*p < 0.01, **p < 0.0001; all histograms represent Mean ± SD; n = 3; three different biological samples from each age group.)