Research Paper Volume 3, Issue 8 pp 768—781

Figure 5. Determination of median lifespan under normal and oxidative stress condition. A. Median lifespan under normal standard condition (PASM without hydrogen peroxide) was analyzed for the wild-type (n=66), ΔPaCatB (n=20), PaCatB_OEx1 (n=29), PaCatB_OEx2 (n=11) and PaCatB_OEx3 (n=14). Neither median lifespan of ΔPaCatB (17.67 d) nor median lifespan of PaCatB over-expression strains (PaCatB_OEx1:18.23 d, PaCatB_OEx2:18.25 d and PaCatB_OEx3:18.5 d) were differentially to the median lifespan of the wild-type (18.48 d). P values were determined using the Superior Performing Statistical Software (SPSS) 16 (SPSS Incorporated). B Median lifespan under oxidative stress (PASM containing 3 mM hydrogen peroxide) was analyzed from the wild-type (n=40), PaCatB_OEx1-3 (n=40). The median lifespan for wild-type was 7.2 d, PaCatB_OEx1:10 d, PaCatB_OEx2:9.72 d and PaCatB_OEx3:9.93 d. Lifespan was 35 % significant increased (p-value: p<0.001). C Median lifespan under oxidative stress (PASM containing 0.75 mM hydrogen peroxide) was analyzed from the wild-type (n=23) and ΔPaCatB (n=27). The median lifespan for wild-type was 13.12 d and for ΔPaCatB 6.7 d. Lifespan was 49 % significant reduced (p-value: p<0.001).