Research Paper Volume 3, Issue 8 pp 754—767

Figure 3. Measurement of ETC complex activities from young, middle aged and old WT and dwarf mouse pectoralis muscle mitochondria. Complex enzyme activities were measured spectrophotometrically as described in Methods. All activity results are averages of 4 assays from the pooled sample ± SEM for each age group. Citrate synthase activities were used to normalize mitochondrial proteins. Activities for young (4-5 months), middle-aged (10-12 months), and old (20-26 months) WT and dwarf pectoralis CI-CV are plotted as follows: (A) CI activity. Coefficients of variance for WT and dwarf were 8.4 % and 19.3% (young), 14.5% and 19.6% (middle-age), and 4.1% and 16.2% (old), respectively. (B) CII activity. Coefficients of variance for WT and dwarf were 6.7% and 3.7% (young), 3.5% and 7.7% (middle-age), and 11.8% and 5.1% (old), respectively. (C) CIII activity. Coefficients of variance for WT and dwarf were 6% and 7.3% (young), 9.6% and 11.3% (middle-age), and 2.8% and 5.7% (old), respectively. (D) CIV activity. Coefficients of variance for WT and dwarf were 3.2% and 5% (young), 4.9% and 5.7% (middle-age), and 3.3% and 8.7% (old), respectively. (E) CV activity. Coefficients of variance for WT and dwarf were 9.9% and 4.5% (young), 18.2% and 14.1% (middle-age), and 2.2% and 7.4% (old), respectively. * - p<0.05 compared to young, ** - p<0.001 compared to young, † - p<0.05 compared to middle-aged, †† -p<0.001 compared to middle-aged, ‡ - p<0.05 compared to WT, and ‡‡ - p<0.001 compared to WT.