Research Paper Volume 2, Issue 10 pp 709—726
Growth signaling promotes chronological aging in budding yeast by inducing superoxide anions that inhibit quiescence
Figure 3. Effects of high glucose (10%) in SC or YPD medium. (A) CLS of wild type cells in SC medium initially containing 2% or 10% glucose. (B) Reproductive capacity after 2 days of medium depletion of wild type, mec1-21 or rad53-21 cells cultured in SC medium containing 10% glucose. (C) Levels of O2- detected by DHE fluorescence in SC cultures of wild type cells at day 3. (D) Levels of H2O2 detected by DHR fluorescence in SC cultures at day 3. (E) Fraction of cells with visible buds in SC cultures at day 3 of medium depletion. (F) CLS of wild type and sch9Δ cells in YPD medium initially containing 2% or 10% glucose. (G) Levels of O2- detected by DHE fluorescence in YPD cultures at day 3. (H) Fraction of cells with visible buds in YPD cultures at day 3.