Research Paper Volume 2, Issue 10 pp 709—726

Figure 2. Constitutive activation of growth signaling pathways shortens CLS in concert with increased O₂^- and less frequent growth of stationary phase cells in G0/G1. (A) Levels of O₂^- detected by DHE fluorescence in wild type and rim15Δ cells at day 3 of medium depletion. (B) CLS of wild type and sic1Δ cells. (C-D) Levels of DHE fluorescence (C) and fraction of cells with visible buds (D) in wild type and sic1Δ cells. Measurements depicted in panels C and D were made at day 3 of medium depletion. E. DNA content of wild type and sic1Δ cells at day 3 of medium depletion. (F-H) Effects of inactivating Snf1p on CLS (F), fraction of cells with visible buds (G) and levels of O₂^- detected by DHE fluorescence. (H) Measurements depicted in panels G and H were made at day 3 of medium depletion.