Figure 4. c-Fos expressions in stress-related neurons after icv administration of nesfatin-1. (A-D) Double-immunohistochemical staining of c-Fos (black) together with CRH (brown) in PVN (A), with tryptophan hydroxylase (TPH) in DR (B), with TH in NTS (C) and LC (D) after icv saline (left panels) and nesfatin-1 (right panels). (E) Incidence of c-Fos-IR neurons in CRH-IR, TPH-IR and TH-IR neurons in the corresponding areas, respectively. Scale bars, 100 μm. 4V, forth ventricle. White arrows indicate CRH-, TPH- or TH-IR neurons, black arrows c-Fos-IR neurons, and blue arrows both-IR neurons. *p < 0.05 vs. saline.