Figure 8. PARPi functions in hESC and induces repair foci but does not affect NHEJ. (A) PARPi inhibits PARP in hESCs. Images (top panel) and graphical depiction (bottom panel) of γ-H2AX foci in hESCs and NPs aftercells were treated with PARPi at 3 μM for 16 h. (B) PARPi does not affect NHEJ in hESCs. BG01V/NHEJ-red cells were treated with PARPi for 16 h and collected at 24 h after Ad-SceI infection. (Columns) Relative qPCR levels was normalized to β-actin; (Error bars) SEM of three samples. No statistical significance was found between treated and untreated sample.