The ASK1-Signalosome regulates p38 MAPK activity in response to levels of endogenous oxidative stress in the <i>Klotho</i> mouse models of aging

C-C Hsieh; Makoto Kuro-o; Kevin P. Rosenblatt; Reynolds Brobey; John Papaconstantinou

doi:doi:10.18632/aging.100194

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Research Paper Volume 2, Issue 9 pp 597—611

The ASK1-Signalosome regulates p38 MAPK activity in response to levels of endogenous oxidative stress in the Klotho mouse models of aging

Figure 3. Binding of 14-3-3 and Trx to ASK1 is part of the inhibitory ASK1-signalosome complex. Assembly of the inhibitory ASK1-signalosome involves the binding of ASK1 to 14-3-3 [56]. Using anti-ASK1 for co-immunoprecipitation analyses we demonstrated (A, C) 14-3-3 and (B, D) Trx are complexed with ASK1. Furthermore, the data show that the ASK1-Trx-14-3-3 complex, a characteristic of the inhibitory ASK1-signalosome, is significantly elevated in the Klotho overexpressing model. (E) Treatment of the liver extracts with the thiol-reacting reagent shows a higher level of reduced Trx in the Klotho overexpressing liver.