A new dominant peroxiredoxin allele identified by whole-genome re-sequencing of random mutagenized yeast causes oxidant-resistance and premature aging

Bernd Timmermann; Stefanie Jarolim; Hannes Rußmayer; Martin Kerick; Steve Michel; Antje Krüger; Katharina Bluemlein; Peter Laun; Johannes Grillari; Hans Lehrach; Michael Breitenbach; Markus Ralser

doi:doi:10.18632/aging.100187

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Research Paper Volume 2, Issue 8 pp 475—486

A new dominant peroxiredoxin allele identified by whole-genome re-sequencing of random mutagenized yeast causes oxidant-resistance and premature aging

Figure 3. Ectopic expression of TSA1-B7 mediates CHP resistance. (A) Candidate genes HOT1, CLC1, DON1, and TSA1 were amplified from the K6001-B7 genome, subcloned, and ectopically expressed in the K6001 parent. Transformants expressing TSA1-B7 were viable on CHP media. (B) CHP resistance is specific to the expression of TSA1-B7. Centromeric plasmids encoding wild type TSA1⁺ and its B7 form were transformed into K6001. The additional copy of TSA1⁺ had no effect on CHP resistance in K6001 (left) and S288c/BY4741 (right).