Figure 3 — A new dominant peroxiredoxin allele identified by whole-genome re-sequencing of random mutagenized yeast causes oxidant-resistance and premature aging

Research Paper|Volume 2, Issue 8|pp. 475–486

A new dominant peroxiredoxin allele identified by whole-genome re-sequencing of random mutagenized yeast causes oxidant-resistance and premature aging

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Figure 3.Ectopic expression of TSA1-B7 mediates CHP resistance.(A) Candidate genes HOT1, CLC1, DON1, and TSA1 were amplified from the K6001-B7 genome, subcloned, and ectopically expressed in the K6001 parent. Transformants expressing TSA1-B7 were viable on CHP media. (B) CHP resistance is specific to the expression of TSA1-B7. Centromeric plasmids encoding wild type TSA1⁺ and its B7 form were transformed into K6001. The additional copy of TSA1⁺ had no effect on CHP resistance in K6001 (left) and S288c/BY4741 (right).